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Home Shop 4esci Gradient PCR Optima 96
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Precipetteâ„¢ Pipettes - Single-channel Adjustable Volume Pipette
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Gradient PCR Optima 96

Gradient PCR are essential laboratory equipment for researchers who run polymerase chain reaction (PCR) for sequencing, cloning, genotyping, mutagenesis, and many other applications. It features precise PCR annealing temperature gradient to easily optimize PCR assays in a single run.

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Description

FEATURES

  • Six Independent temperature Blocks with imported TEC modules provide precise Temperature control for PCR optimization
  • Gradient PCR thermal cycler with excellent thermal gradient temperature accuracy.
  • Independent front and rear air inlet and outlet duct design, which allows instruments to be placed close to each other, saving laboratory space.
  • Ultra large fin-type heat sink with intelligent fan speed control technology provides low-noise heat dissipation.
  • Flexible lid: Heated lid automatically adjusts to fit every PCR tube, creating pressure that prevents sample evaporation, to ensure reproducible results in every run.
  • The Lid comes with Intelligent auto-heating off function to reduce non-specific amplification by turning off the heating when the temperature of sample blocks is below 30°C.
  • Faster Heating and cooling Rates (Maximum heating rate >5°C/s, maximum cooling rate >4°C/s)
  • Provides excellent temperature uniformity and the gradient layout reduces edge effect
  • Comes with USB interface at the front for easy gradient PCR protocol import & export. Also, Device software can be upgrade through USB
  • Gradient PCR machine comes with Multiple Accounts and Access rights (Administrator, User Accounts, Visitor Accounts)
  • High resolution, 7-inch LCD touchscreen panel for easy operation and monitoring
  • Touchdown (TD) PCR Mode and Long Range PCR Modes are available
  • Compatible with universal PCR tubes, PCR 8 strips plates and 96-well PCR plates
Specifications

Specifications

TEMPERATURE CONTROL

 

Model Optima96
PN PR122
Sample Capacity 0.2mL PCR Tubes, 0.2mL 8-tube Strips,

0.2mL 96-well PCR Plate (non-skirted/semi-skirted)

PCR reaction volume 10-100 µl
Heating lid temperature 30-110°C
Max. block heating Rate 5°C/s
Max. block cooling Rate 4 °C/s
Temperature uniformity <0.5°C (20s after reaching 95°C )
Sample temperature range 4°C-100°C
Temperature accuracy ±0.25°C (35°C – 99.9°C)
Technology  (heating/Cooling) Semiconductor Peltier technology, 6 independent  blocks
Display resolution 0.1°C

GRADIENT

 

Maximum temperature

difference between blocks

 

25°C (max. 5°C between two adjacent blocks)

Temperature difference between

two adjacent blocks

 

0.1°C-5.0°C

Gradient temperature accuracy ±0.25°C (35°C – 99.9°C)
Gradient temperature uniformity <0.5°C (20s after reaching 95°C)

PROGRAM FUNCTION

 

LCD display 7″ LCD touch screen, real-time display of PCR process,

can be used while wearing gloves

Max. number of steps 55
Time increment / decrement 1-120s, for long Range PCR experiment
Temperature increment / decrement 0.1°C-10.0°C,  for Touchdown PCR
Maximum number of cycles 999

OTHER FEATURES

 

Tm value calculation function Yes
Automatic pause/power-off protection Yes
Communication interface USB 2.0
Storage External USB Drive and Internal Memory
 

Program storage capacity

Up to 1,000 programs in the instrument,

Unlimited storage with USB flash drive

POWER SUPPLY

 

Input power 100-240V, 50-60Hz
Max. power 750 V A
Max. current 10A
Noise <55dB
Shipping & Delivery
Category: 4esci
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